downs syndrome (ds) is one of the most common chromosomal abnormalities (1 in 700-1000 births) and one of the main causes of mental retardation. therefore, diagnosis and prevention of live-born children with ds is a principle priority for the iranian ministry of health. this study was performed to evaluate the application of real-time qpcr technique for rapid diagnosis of ds. about 15-20 millil...

in order to estimate responses of growth rate and growth hormone of clarias gariepinus to stocking density, fish (initial weight 30.71 ± 0.89 g) were stocked in 120-l tanks at densities of 35, 65, 95, and 125 kg/m3 for 60 days. on day 30 and 60, the fish growth rates were measured, pituitary growth hormone (gh) mrna levels and serum gh concentrations were examined by real-time qpcr and elisa te...

lentiviral vectors (lvs) are useful vehicle for genetransfer to dividing and non-dividing cells and genetic manipulations. however, the use of lentiviruses in studies requires an accurate titration technique.quantitative real-time pcr (qpcr) is a sensitive technique for the indication and quantitation of retrovirals particles. in this study, we used the qpcr for lentiviral vector titeration. th...

This study aimed to verify the reliability of the 7 tissue differentially methylated regions used in the methylated DNA immunoprecipitation (MeDIP) real-time quantitative polymerase chain reaction (real-time qPCR) based approach of fetal DNA in maternal blood to diagnosis of fetal trisomy 21. Forty pregnant women with high risk pregnancy who were referred after first or second trimester screeni...

Real time-qPCR is the most reliable method for evaluation of mRNA expression levels. However, to obtain accurate results, selection of suitable reference genes is necessary for normalizing the real-time qPCR data. The aim of this research was to validate the expression stability of three potential reference genes (ACTB, GAPDH and UXT) in milk somatic cells of Holstein dairy cattle under differe...